The Effect of Morphine Delta Receptor Activity on Ischemic Postconditioning in Lung Ýschemia Reperfusion Ýnjury

Objective: In the context of the physiopathology of lung damage due to ischemia and reperfusion injury, we aimed to demonstrate the effects of the addition of morphine sulphate to ischemic post-conditioning protocol.
Methods: In the present study, 48 Wistar albino female rats were employed. Group 1 was accepted as the Sham group that underwent thoracotomy through the fifth left intercostal space. IR group: thoracotomy and ischemia reperfusion period. IRPC group: thoracotomy, ischemia reperfusion period and ischemic post-conditioning. In IRPC3 and IRPC30 groups, in addition to ischemic post-conditioning different doses of morphine sulfate (3μmol and 30μmol) was administered. TNF- α, IL-1, and IL- 10 levels were measured in biochemical assessment of the lung tissue samples obtained.
Results: TNF-α and IL-1 (pro-inflammatory cytokines) have lower values and IL-10 (antiinflammatory cytokine) have higher values both in the groups which have been subject to postconditioning and morphine. TNF-α and IL-1 levels in lung tissue were statistically significant between the IRPC3 group and the IR and IRPC groups. In addition, IL-10 level in lung tissue were statistically significant between the IRPC3 group and the IRPC group.
Conclusion: In the present study conducted with experimental animals where morphine was also injected besides ischemic PC protocols, statistically significant differences were determined in the lung tissue analyses when we compared pro-inflammatory and anti-inflammatory cytokine values. We firmly believe that adding morphine to the lung transplantation protocols and post conditioning will decrease ischemia reperfusion damage.